External quality assessment by European mycobacterial laboratories: results of AFB microscopy and identification rounds
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE(2022)
摘要
We analyzed mycobacterial stain, culture and identification EQA data from altogether 134 laboratories in 15 mainly European countries over a 4-year period. The aim was to get an overview of the performance and methods and identify diagnostic challenges. The overall success rates for staining and identification were 94% and 91%. The false negative rate for staining was significantly higher for the medium positive than the strong positive slides (11% vs 4%). The false positive rate on negative slides was 10%, indicating contamina-tion issues. The overall success of M. tuberculosis detection was high with error rates ranging from 0.7% to 1.2%. Pre-or postanalytical errors accounted for most of the unsuccessful responses. The detection of nontu-berculous mycobacteria (NTM) was less consistent; accurate species identification depended on the assays used. Only 19% of participants performed species level identification for NTMs, 47% detected the presence NTMs while 21% focused on ruling out TB. (c) 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
更多查看译文
关键词
Mycobacterium tuberculosis, nontuberculous mycobacteria, EQA, stain, identification, acid-fast bacilli
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要