Structural features of free N-glycans in alpha 1,3/4-fucosidase-deficient Arabidopsis thaliana: deletion of alpha 1,3/4-fucosidase activity induced accumulation of plant complex type GN1 free N-glycans

Deletion of alpha-1,3/4-fucosidase activity in Arabidopsis thaliana resulted in the accumulation of GN1-type free N-glycans with the Lewis a epitope (GN1-FNG). This suggests that the release of alpha-fucose residue(s) may trigger rapid degradation of the plant complex-type (PCT) GN1-FNG. The fact that PCT-GN1-FNG has rarely been detected to date is probably due to its easier degradation compared with PCT-GN2-FNG.