Tissue Fixation with a Formic Acid-Deprived Formalin Better Preserves DNA Integrity over Time.

Enrico Berrino,Laura Annaratone,Paolo Detillo,Dora Grassini,Alberto Bragoni,Anna Sapino,Benedetta Bussolati, Giovanni Bussolati,Caterina Marchiò

Pathobiology : journal of immunopathology, molecular and cellular biology（2022）

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摘要

Although DNA fragmentation in FFPE tissues is a multifactor process, we showed that the removal of formic acid is responsible for a significant improvement in DNA preservation.

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关键词

Acid-deprived formalin,DNA integrity,Next-generation sequencing,Tissue fixation

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