Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

•Both post-ripening stages and banana varieties contribute to metabolite variation.•AuNP-assisted LDI-MSI was firstly used in mapping functional metabolites in pulps.•AAs and monoamines exclusively accumulated in the middle region near the seed zone.•Monosaccharides locate in whole pulps but enrich in the intermediate microregion.•Di/trisaccharides exhibit different accumulation patterns as monosaccharides.