Isolation, Characterization and Anticomplementary Activity of Polysaccharides from the Rhizomes of Belamcanda chinensis (L.) DC.
CHEMISTRY & BIODIVERSITY(2022)
摘要
The polysaccharides from the rhizomes of Belamcanda chinensis (L.) DC. (BCPs) were obtained by optimal water extraction (extraction temperature 84 °C, liquid to solid ratio 42 mL/g and extraction time 100 min), the extraction yield of BCPs was 23.01±0.27 % (n=3). Furthermore, two novel polysaccharides (BCP-A1 and BCP-B1) were purified by column chromatography. The BCP-A1 (6.0820×104 kDa) was composed of β-D-Manp-(1→, β-D-Glcp-(1→, →4)-α-D-Galp-(1→ and →3,4)-β-D-Galp-(1→, and BCP-B1 (2.2744×104 kDa) was composed of →5)-α-L-Araf-(1→, β-D-Manp-(1→, β-D-Glcp-(1→, →4)-α-D-Glcp, →4)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→ and →3,4)-β-D-Galp-(1→. In anticomplementary experiments, BCP-A1 (CH50 : 0.009±0.003 mg/mL; AP50 : 0.015±0.003 mg/mL) and BCP-B1 (CH50 : 0.004±0.001 mg/mL; AP50 : 0.028±0.005 mg/mL) exhibited potent anticomplementary activity, and acted on C2-, C4- and Factor B components. Our study provides a foundation for BCP-A1 and BCP-B1 as potential complement inhibitors to treat diseases involving with excessive activation of the complement system.
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关键词
Belamcanda chinensis, polysaccharides, characterization, anticomplementary activity
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