Lipoxin A4 (LXA4) Improved LPS Induced Preeclampsia via Regulation Nrf2-ARE Signaling Pathway In Vitro

The aim is to discuss effects and mechanism of LXA4's improvement effect in LPS induced preeclampsia in vitro study. Using LPS induced HTR-8/SVneo to make preeclampsia cell model. Evaluating ROS, MDA, SOD and GSH in difference groups. It was measured cell proliferation, apoptosis, invasion and migration by CCK-8, flow cytometry, transwell and wound healing assay in difference groups. Relative proteins including Nrf2 and HO-1 were measured by WB assay. With LPS stimulating, ROS and MDA were significantly up-regulation and GSH and SOD levels were significantly depressed, meanwhile, cell proliferation was significantly downregulation with cell apoptosis significantly increasing, invasion cell number and wound healing rate were significantly depressed (p < 0.001, respectively) with Nrf2 and HO-1 proteins levels were significantly down-regulation (p < 0.001, respectively) in LPS groups. LXA4 supplement, cell biological activities were significantly up-regulation, however, after Nrf2 knockdown, the LXA4 treatment effects were disappear. LXA4 has effects to improve LPS induced preeclampsia to enhance cell biological activities by regulation Nrf2/HO-1 pathway in vitro study.