Qualitative and semi-quantitative phase analysis of TiO2 thin layers by Raman imaging

•We presented a new perspective for the qualitative and semi-quantitative analysis of TiO2 oxides phase concentration.•For the first time, we applied Raman imaging to determine phase distribution and estimated rutile/anatase concentration in TiO2 thin layers.•Rutile and anatase phase mixture has beneficial properties to create a permanent tissue-implant connection.•We evaluated the distribution of TiO2 layer components using K-means Cluster Analysis (KMC) and calculations bands' relative intensity.•We demonstrated the advantages of Raman imaging in calculating the surface composition essential for biomaterials surface preparation and evaluation.