The complete chloroplast genome sequence of Gentiana triflora and comparative analysis with its congeneric species

Gentiana triflora is an important medicinal plant in China with economic and medicinal value. Here, we report the complete chloroplast sequences of G. trifloral. The cp genome of G. triflora of 149, 125 bp contains 130 unique genes, including 85 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The analysis of repeat showed that palindromic had the highest frequency. Besides, a total number of 45 SSR were identified, most of which were mononucleotide adenine-thymine. Comparative genome analysis of Gentiana species revealed that the pair of the inverted repeat was more conserved than the single-copy region. This analysis resulted in identification of 8 hypervariable regions (trnH-GUG, trnG-UCC-intron, atpI, trnD- GUC, trnL-UAA, rpl32-trnL-UAG, petA and ycf1). Phylogenetic analysis revealed that G. triflora was most closely related to Gentiana manshurica. In conclusion, this study enriched the genomic resources of the Gentiana genus and provided a basis for evolution and phylogeny analyses.