HPLC analysis of vitexin and isovitexin content changes during mung bean germination

In order to study the changes in the content of vitexin and isovitexin in different varieties of mung beans during the germination process, a method based on high performance liquid chromatography for the determination of vitexin and isovitexin in mung beans was established. The study used four varieties of mung beans as the study objects, with acetic acid water and acetonitrile as mobile phases, and the diode array detector was used for measurement. Under the optimized conditions, the linear relationship of the nine kinds of vitexin and isovitexin were good in a certain mass concentration range, the correlation coefficient was greater than 0.995, the detection limits of vitexin and isovitecin were 0.04173 μg/mL and 0.023255 μg/mL, the recovery of the method was 80.05–101.54%, the relative standard deviation less than 5.0%. The results showed that vitexin and isovitexin showed a downward trend during the germination process of mung bean. Compared with the existing methods, this method is sensitive, fast and applicable, and can effectively determine the content changes of vitexin and isovitecin during the germination process of mung bean.