Complete genomic sequence of an isolate of plant-associated genomovirus 12 (genus Gemycircularvirus ) from open–field tomatoes in Brazil

Using a next–generation sequencing (NGS) approach, we identified a gemycircularvirus ( Genomoviridae ) in association with foliar samples of open–field tomatoes in Brazil. The viral ssDNA genome (2,189 nucleotides) was amplified and Sanger–sequenced. BLASTn and sequence demarcation tool (SDT) analyzes showed that the virus from tomato shares 99% identity with a virus tentatively named as plant–associated genomovirus 12 from Larrea tridentata (Zygophyllaceae). SDT analyzes of a wide array of Genomoviridae sequences indicated this virus as a member of the genus Gemycircularvirus . This the first report of a gemycircularvirus in natural association with a species of the Solanaceae.