Synergistic Effect of Silver and Zno Nanoparticles Green Synthesized by Vitis Vinifera Stem Extract with Ampicillin against Some Pathogenic Microbes

The increasing of multiple drug resistance pathogens necessitated extensive research for new safe and effective therapeutic agents. Vitis vinifera stem (VVS) hydroalcoholic extract beside its biosynthesized metallic nanoparticles silver (VVSAgNPs) and zinc oxide (VVSZnONPs) were tested against pathogenic microbes namely; B. subtilis ATCC6633, S. aureus ATCC29213, E. coli ATCC25922, S. typhimurium ATCC14028 and the fungus C. albicans ATCC1023. HPLC/MS/MS analysis of VVS extract led to identification of 31 compounds. The major compounds were malic, quinic and vanillic acids, catechin gallate and reservatrol and its dimer viniferin beside 11 fatty acid and terpene. The VVSAgNPs and VVSZnONPs were characterized using UV, FT-IR, TEM and zeta potential measurements. All the tested samples gave antimicrobial activity against all tested pathogens. VVSAgNPs were the most effective either alone or as synergists with the antibiotic ampicillin and giving the highest growth inhibition with the antibiotic at 1xMIC and 3xMIC. Also, the reduction of microbial population was more than 3 log for B. subtilis, E. coli and 2.3 log for C. albicans at 4xMIC using VVAgNPs. Thus, VVS hydroalcoholic can be used in food industry and pharmaceutical industry as antimicrobial agent while silver nanoparticles could be used as a promising disinfectant with long stability on surface materials that could be used in the hospitals as a disinfectant spray for preventing emergence of microbial resistance.