Isolation and partial characterization of bacteriophages infecting Vibrio harveyi from shrimp farm effluent water
Aquaculture International(2022)
摘要
Bacteriophage isolated from the semi-intensive culture of Pacific white leg shrimp Litopenaeus vannamei infects the luminous bacteria Vibrio harveyi . Lytic activity and lytic spectrum results revealed that the isolated phage had strong lytic activity in V. harveyi , V. parahaemolyticus , and V. vulnificus . Biofilm inhibition activity was performed against different pathogenic vibrios on high-density polyethylene (HDPE) template and the result revealed that the phage effectively inhibited the biofilm formation in V. harveyi . Spectrophotometric assay performed for lytic activity of the isolated phage in V. harveyi liquid culture showed that the phage significantly decreased the V. harveyi cell densities at different time intervals ( P < 0.05). To study the stability of phage at different temperature and pH revealed that the phage withstands the temperature ranged between 40 and 70 °C and the pH of 4 and 9 at a significant level ( P < 0.001). One-step growth curve depicted that the burst size gradually increased to a significant level and reached the maximum of 90% at 180 min ( P < 0.05). This study concluded that the isolated phage had specific activity against pathogenic V. harveyi infections.
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关键词
Bacteriophage,Biofilm,Lytic spectrum,Vibrio harveyi,Shrimp farm effluent
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