Establishment and optimization of agrobacterium-mediated transformation in blueberry (Vaccinium species)

•The combination of two mg/l zeatin and one mg/l indole-3-butyric acid is critical for the regeneration of Vaccinium leaves.•The diploid Vaccinium reticulatum ‘Red Button’ has the highest efficiency of callus formation and adventitious bud regeneration.•Agrobacterium-mediated transformation efficiency in ‘Red Button’ is about 12.82%.