(Digital Presentation) Highly Sensitive Detection of Cell Endogenous Hydrogen Sulfide Based on in-Situ Dynamic Reaction of Ag NPs

The performance of nanoscale sensors can often provide insights into the extremely subtle differences between biomolecules. Meanwhile, several innovative signal transduction pathways of biosensing systems have attracted a lot of attention, but most of these are always driven by a single mechanism[1]. Herein, we demonstrate a biosensor for in situ quantification of active sulfide in the cell that utilizes the electrochemical solid-state phase transformation mechanism of the self-assembled silver nanoparticles[2], which is immobilized on the surface of the gold microelectrode through Raman signal molecules (4-aminothiophenol, 4-ATP). Taking the detection of cell endogenous hydrogen sulfide (H2S) as an example, Ag NPs could first slowly react with local H2S. Under the occurrence of the electrochemical oxidation reaction, a large number of silver ions (Ag+) are released to quickly react with H2S. Subsequently, the execution of the reduction reaction brings the complex complexes to the electrode surface. The electrochemical solid-phase transfer mechanism has a fast reaction rate, and the shift of the redox peak of Ag NPs and the strength of the Raman spectrum of 4-ATP can quickly and accurately quantitatively analyze the cell endogenous H2S with two channels, and the detection limit can reach nanomolar level. Going beyond conventional biosensing, the elaborate dynamic coupling of multiple mechanisms in a micro/nanoscale interface may open a new approach for future highly sensitive biosensing. References [1] H Zhou, G Ran, JF Masson, C Wang, Y Zhao, Q Song, Analytical Chemistry 2018, 90(5), 3374-3381 [2] H Zhou, R Yu, G Ran, S Moussa, Q Song, J Mauzeroll, JF Masson, Sensors and Actuators B: Chemical 2020, 319, 128315