A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction

Myriam Belén González Viacava,Carlos Leónidas Leiva, María Eugenia Tavarone,José Christian Dokmetjian,Osvaldo Cascone,Pablo Chacana,Matías Fingermann

SSRN Electronic Journal（2022）

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摘要

•Strong anion exchangers are superior to week anion exchangers for Fab’ purification.•Ultrafiltering pepsin treated samples is critical for chromatographic performance.•Highly purified Fab’ IgY fragments were obtained in three cost-effective steps.•The process is scalable to industrial level.

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关键词

Egg yolk antibodies,Water-soluble fraction,Fab’ fragment,Anion-exchange chromatography,Purification

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