Genotype VII.1.1-Based Newcastle Disease Virus Vaccines Afford Better Protection against Field Isolates in Commercial Broiler Chickens

Simple Summary Controlling genotype VII Newcastle disease virus (NDV) is challenging, especially in endemic countries. Genetic engineering was used to develop recombinant vaccines against NDV (rNDV). The close genetic relationship with circulating viruses can better protect against field NDV challenges. This study evaluated two commercial rNDV genotype VII.1.1 vaccines based on the LaSota strain backbone or VG/GA strain backbone compared to conventional genotype II vaccines. Both vaccines induced a protective immune response; however, GII-based vaccines failed to prevent virus shedding efficiently. Additionally, the noticeable superior performance of the rNDV vaccine based on the VG/GA strain backbone may be attributed to the enterotropic nature of the VG/GA strain, which makes it replicate more efficiently in both the respiratory and intestinal tracts of chickens. Future research needs to evaluate the cell-mediated immune response induced by the rNDV GVII vaccines to understand their mechanism better mediating the mucosal immunity. This study evaluated the efficacy of live and inactivated conventional GII LaSota and recombinant GVII Newcastle disease vaccines in commercial broilers. The experimental groups (G2-G7) were vaccinated on day 7 and day 21 of age with live vaccines from the same vaccine type "GII LaSota, GVII vaccine (A), GVII vaccine (B)" via eye drop; however, G3, G5, and G7 received a single dose from inactivated counterpart vaccines subcutaneously on day 7 of age. Vaccine efficacy was evaluated based on elicited humoral immunity, clinical protection, and reduction in virus shedding after challenge with virulent GVII 1.1. strain. Results demonstrated that live and inactivated recombinant GVII vaccine based on VG/GA strain backbone elicited superior protection parameters (100% protection). Although the conventional GII LaSota live and inactivated vaccination regime protected 93.3% of vaccinated birds, the virus shedding continued until 10 DPC. The post-vaccination serological monitoring was consistent with protection results. The study concludes that conventional GII ND vaccines alone are probably insufficient due to the current epidemiology of the GVII 1.1 NDV strains. Our findings further support that protection induced by recombinant GVII 1.1. ND vaccines are superior. Interestingly, the efficacy of recombinant ND vaccines seemed to be influenced by the backbone virus since the VG/GA backbone-based vaccine provided better protection and reduced virus shedding.