Feasibility and reliability of measures of bioactive lipids in human plasma and nasal mucosa

•Analysis of bioactive lipids is increasingly useful in clinical studies.•There is a need for reliable, non-invasive and easy-to-use sampling methods.•Nasal mucosal filter samples was suitable for the analysis of bioactive lipids.•Measures of reliability varied in samples from both plasma and nasal mucosa.•Arachidonic acid derivatives show better reliability regardless of matrix.