Size Tunable Silver Nanoparticles and Their Antimicrobial Activity Against Staphylococcus Aureus and Escherichia Coli in Control of Bovine Mastitis

Silver nanoparticles (AgNPs) possess unique optical, electrical, thermal, high electrical and biological properties, scrutinizing them as a greater effective antimicrobial molecule against a wide variety of bacteria. This prompts us to carry out this current experiment to synthesize size-tunable AgNPs and to them characterize for use as an effective antimicrobial agent against Staphylococcus aureus and Escherichia coli of bovine mastitis. In this study, AgNPs were synthesized in both chemical and green methods and characterized with UV/VIS Spectroscopy, Transmission Electron Microscopy, Dynamic Light Scattering, and Fourier Transform Infrared Spectroscopy. Further, the antimicrobial activity of all synthesized AgNPs was tested against S. aureus and E. coli with a comparative study for commonly available commercial antibiotics. The comparative antimicrobial study revealed that green synthesis AgNP -2(1:4) and AgNP-5 (1:1) showed a higher zone of inhibition than chemically synthesized AgNP -1 (1%) and AgNP-4 (2%) and also than commercial antibiotics irrespective of the organism. Further, it has resulted that the antimicrobial effect of AgNPs has higher against S. aureus than the E. coli. This study concluded that the green synthesis AgNPs would be a suitable alternative in place of commercially available antibiotics against S. aureus and E. coli in control of bovine mastitis.