A High‐Throughput Continuous Spectroscopic Assay to Measure the Activity of Natural Product Methyltransferases

Hadas Simon-Baram, Steffen Roth, Christina Niedermayer, Patricia Huber,Melanie Speck, Julia Diener,Michael Richter,Shimon Bershtein

ChemBioChem(2022)

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摘要
E.coli BL21(DE3) cells carrying the pMHT1 TmMtaD expression vector are a kind gift from Prof. Amir Aharoni and Prof. Dan Levi, Ben-Gurion University of the Negev, Israel. The plasmid pET28 carrying the MjDadD gene was a kind gift from Prof. Jennifer Andexer, University of Freiburg, Germany. The authors acknowledge the Federal Ministry of Education and Research (BMBF, Germany) (161B0626 A) for M. R. and the Israel Ministry of Health (ERA-NET 5317) for S. B. for funding in frame of the 17-ERACoBioTech as part of the project BioDiMet: Methyl Transferases for the Functional Diversification of Bioactives.
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关键词
biocatalysis,high-throughput screening,natural products,small molecule methyltransferases
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