Use of a sample-to-result shotgun metagenomics platform for the detection and quantification of viral pathogens in paediatric immunocompromised patients

Divya Shah,Julianne R Brown,Jack C D Lee,Meredith L Carpenter, Gavin Wall,Judith Breuer

Journal of Clinical Virology Plus（2022）

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摘要

•The Galileo Viral Panel metagenomic sequencing platform was compared to singleplex qPCR for the detection and quantification of DNA viruses in immunocompromised paediatric patients.•Galileo had high qualitative and quantitative agreement with qPCR.•Galileo was able to detect additional viruses not targeted in routine testing.

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Metagenomics,Transplant viruses,Next-generation sequencing,Paediatric viral infection,qPCR

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