Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children
European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry(2022)
摘要
Purpose The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR). Materials and methods Dental plaque samples were collected from 57 subjects including S-ECC ( n = 33) and CF ( n = 24) patients. The two groups were matched in terms of age, gender, and socioeconomic status. After deoxyribonucleic acid (DNA) extraction, SYBR Green real-time PCR was used to quantify CMV DNA. Results The CMV DNA was detected in seven patients in the S-ECC group. The mean of decayed, missing, and filled tooth surfaces (dmfs) was 11.90 ± 5.95 in the S-ECC group. There was no significant difference in gender between the two groups. There was a significant relationship between CMV positivity in children and the S-ECC group ( p = 0.043). Conclusion Results of this study suggest a relationship between the presence of CMV in the oral cavity and S-ECC. We can design clinical trials to confirm these results and improve children's dental care.
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关键词
Cytomegalovirus,Severe early childhood caries,Real-time PCR
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