From Methylome to Integrative Analysis of Tissue Specificity.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.
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关键词
Catharanthus roseus,DNA methylation,Methylome pipeline,Ovation Methyl-seq,Whole-Genome Bisulfite Sequencing (WGBS)
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