Mechanism of trypsin activation by pulsed electric field treatment revealed based on chemical experiments and molecular dynamics simulations

Yinli Li,Zihan Yuan,Yuanhong Gao,Zhijie Bao,Na Sun,Songyi Lin

Food Chemistry（2022）

引用 9|浏览3

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摘要

•PEF treatment significantly increased the catalytic rate of trypsin.•PEF treatment tended to order the spatial conformation of trypsin.•PEF treatment improved the stability of trypsin binding to the substrate.•The mechanism that PEF treatment increased trypsin activity was revealed.

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关键词

Trypsin,Activation,Pulsed electric field,Conformational changes,Molecular dynamics simulation,Molecular docking

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