Microfluidic chip interfacing microdialysis and mass spectrometry for in vivo monitoring of nanomedicine pharmacokinetics in real time

•A microfluidic chip coupling microdialysis sampling with mass spectrometry analysis.•Free drug concentrations are accurately monitored online.•Pharmacokinetics of nanomedicines are profiled with high temporal resolution.•High drug extraction recovery is obtained by using PEG 20,000 as an anti-foulant.