Elevated CD4(+) T-cell glucose metabolism in HIV plus women with diabetes mellitus

Objective: Immune dysfunction and chronic inflammation are characteristic of HIV infection and diabetes mellitus, with CD4(+) T-cell metabolism implicated in the pathogenesis of each disease. However, there is limited information on CD4(+) T-cell metabolism in HIV+ persons with diabetes mellitus. We examined CD4(+) T-cell glucose metabolism in HIV+ women with and without diabetes mellitus. Design: A case-control study was used to compare CD4(+) T-cell glucose metabolism in women with HIV with or without diabetes mellitus. Methods: Nondiabetic (HIV+DM-, N = 20) or type 2 diabetic HIV+ women with (HIV+DM+, N = 16) or without (HIV+DMTx+, N = 18) antidiabetic treatment were identified from the WIHS and matched for age, race/ethnicity, smoking status and CD4(+) cell count. CD4(+) T-cell immunometabolism was examined by flow cytometry, microfluidic qRT-PCR of metabolic genes, and Seahorse extracellular flux analysis of stimulated CD4(+) T cells. Results: HIV+DM+ displayed a significantly elevated proportion of CD4(+) T cells expressing the immunometabolic marker GLUT1 compared with HIV+DMTx+ and HIV+DM- (P = 0.04 and P = 0.01, respectively). Relative expression of genes encoding key enzymes for glucose metabolism pathways were elevated in CD4(+) T cells of HIV+DM+ compared with HIV+DMTx+ and HIV+DM-. T-cell receptor (TCR)-activated CD4(+) T cells from HIV+DM+ showed elevated glycolysis and oxidative phosphorylation compared with HIV+DM-. Conclusion: CD4(+) T cells from HIV+DM+ have elevated glucose metabolism. Treatment of diabetes mellitus among women with HIV may partially correct CD4(+) T-cell metabolic dysfunction.