Association of diaphragm thickness and echogenicity with age, sex and BMI in healthy subjects

Diaphragm ultrasound is increasingly used in the diagnosis of diaphragm dysfunction and to guide respiratory management in patients with neuromuscular disorders and those who are critically ill. owever, the association between diaphragm ultrasound variables and demographic factors like age, sex and body mass index (BMI) are understudied. Such relationships are important for correct interpretation of normative values and comparison with selected patients groups. The aim of this study is to determine the associations between diaphragm ultrasound variables and subject characteristics.B-mode ultrasound was used to image the diaphragm at the zone of apposition in 83 healthy subjects. Diaphragm thickness at resting end-expiration (Tend-exp ), diaphragm thickness at maximal end-inspiration (Tmax-insp ), diaphragm thickening ratio (Tmax-insp /Tend-exp ), and diaphragm echogenicity were measured. Multivariate linear regression was used to explore the associations between diaphragm ultrasound variables and subject characteristics.Tend-exp , Tmax-insp and thickening ratio do not change with age whereas diaphragm echogenicity increases with age. The thickening ratio had a weak negative association with BMI, while Tend-exp was positively associated with BMI. Men had a larger Tend-exp and Tmax-insp than women (Tend-exp 1.6±0.5 and 1.4±0.3 mm; p=0.011, Tmax-insp 3.8±1.0 and 3.2±0.9 mm; p=0.004), but similar thickening ratios.Diaphragm thickness, thickening and echogenicity measured with ultrasound are associated with factors such as age, BMI and sex. Therefore, subject characteristics should be considered when interpreting diaphragm ultrasound measurements. In the absence of normative values, matched control groups are a prerequisite for research and in clinical practice. This article is protected by copyright. All rights reserved.