Enantiomer fraction evaluation of the four stereoisomers of second-generation anticoagulant rodenticides in biological matrices with polysaccharide-based chiral selectors and liquid chromatography tandem mass spectrometry

•Stereoisomers of chiral rodenticides quantified by LC-MS/MS in raptor liver.•Chiral methods optimized for multiple stereogenic centers pesticides.•Chiral chromatographic conditions and selectors systematically tested.•Separation either in HILIC-like or reversed-phase mode depending on mobile phase.