Preliminary Exploration of Plasmon-Enhanced Four-Wave Mixing Imaging and Its Possible Application in Antibody-Drug Metabolism in the Body

In this study, several imaging functions, such as two-photon imaging and multi-mode four wave mixing (FWM) imaging, were realized on a single multiphoton spectrometer by the combination of double-output picosecond pulse laser and laser scanning microscope platform. Different nonlinear effects were tested to prove that this system could achieve spectroscopic recording and have excellent imaging performance. We explored the possible application of plasmon-enhanced FWM imaging for the metabolism residues of antibody drugs in a mouse's liver and kidney using 5 nm gold nanoparticles as a probe. This method acts as a tool for conducting the metabolism evaluation of protein drugs in the body.