Gates lab qPCR protocol for Cladocopium and Durusdinium Symbiodiniaceae detection v1

Mariana not provided Rocha De Souza,Shayle Matsuda,Jenna Dilworth,Crawford not provided Drury,Ross Cunning

protocols.io（2020）

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摘要

The purpose of this protocol is to quantify Cladocopium and Durusdinium Symbiodiniaceae cell ratio densities (symbiont to host cell ratios) from individual coral DNA samples using the StepOnePlus™ Real-Time PCR System. It allows for a quick assessment of the symbiont community composition and ratio using targeted primers.

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