Mp29-06 use of autologous renal cells from diseased kidneys for the treatment of renal failure

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder II (MP29)1 Sep 2021MP29-06 USE OF AUTOLOGOUS RENAL CELLS FROM DISEASED KIDNEYS FOR THE TREATMENT OF RENAL FAILURE Sunil George, Mehran Albolbashari, John Jackson, Tamer Aboushwareb, Anthony Atala, and James Yoo Sunil GeorgeSunil George More articles by this author , Mehran AlbolbashariMehran Albolbashari More articles by this author , John JacksonJohn Jackson More articles by this author , Tamer AboushwarebTamer Aboushwareb More articles by this author , Anthony AtalaAnthony Atala More articles by this author , and James YooJames Yoo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002026.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Chronic kidney disease (CKD) occurs when certain conditions cause the kidneys to gradually lose function. For patients with CKD, renal transplantation is the only treatment option that restores kidney function. In this study, we evaluated primary renal cells obtained from diseased kidneys to determine whether their normal phenotypic and functional characteristics are retained, and could be used for cell therapy. METHODS: Renal cells were isolated and cultured from three normal kidneys (NK) and three kidneys from donors with CKD. Primary renal cells from each group were evaluated for cell growth patterns and histo-morphological analyses. To confirm renal specific phenotypes, immunofluorescent staining was performed on the cultured cells using renal cell specific markers. Scanning and transmission electron microscopy were performed for surface and ultrastructural analysis. Glutathione assay, sodium uptake function and albumin uptake assay were performed to evaluate cellular function. RESULTS: Primary renal cells isolated from both normal kidneys (NK) and diseased kidneys (CKD) showed similar phenotypic characteristics and growth kinetics. The expression levels of renal tubular cell markers, Aquaporin-1 and E-Cadherin, and podocyte-specific markers, WT-1 and Nephrin, were similar in both NK and CKD kidney derived cells. Using fluorescence- activated cell sorting (FACS), specific renal cell populations were identified and included proximal tubular cells (83.1 % from NK and 80.3 % from CKD kidneys); distal tubular cells (11.03 % from NK and 10.9 % from CKD kidneys); and podocytes (1.91 % from NK and 1.78 % from CKD kidneys). Ultra-structural analysis using scanning electron microscopy (SEM) revealed microvilli on the apical surface of cultured cells from NK and CKD samples. Moreover, transmission electron microscopy (TEM) analysis showed a similar organization of tight junctions, desmosomes, and other intracellular structures. The Na+ uptake characteristics of NK and CKD derived renal cells were also similar (24.4 mmol/L and 25 mmol/L, respectively) and no significant differences were observed in the protein uptake and transport characteristics of these two cell isolates. CONCLUSIONS: These results show that primary renal cells derived from diseased kidneys such as CKD have similar structural and functional characteristics to their counterparts from a normal healthy kidney (NK) when grown in vitro. This study suggests that cells derived from diseased kidney may be used as an autologous cell source for renal cell therapy, particularly in patients with CKD or end-stage renal disease (ESRD). Source of Funding: Tengion, Inc © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e491-e492 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sunil George More articles by this author Mehran Albolbashari More articles by this author John Jackson More articles by this author Tamer Aboushwareb More articles by this author Anthony Atala More articles by this author James Yoo More articles by this author Expand All Advertisement Loading ...