Covalent and non-covalent coupling of a Au₁₀₂ nanocluster with a fluorophore: energy transfer, quenching and intracellular pH sensing

Eero Hulkko,Tanja Lahtinen,Varpu Marjomäki, Emmi Pohjolainen,Ville Saarnio,Karolina Sokolowska, Ardra Ajitha,Mikael Kuisma,Lauri Lehtovaara,Gerrit Groenhof,Hannu Häkkinen,Mika Pettersson

Nanoscale Advances（2021）

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摘要

A covalently bound hybrid between an atomically precise gold nanocluster and a fluorescent dye is loaded into cells via endocytosis. Acidification of the endosomes activates fluorescence, enabling optical pH sensing inside live cells.

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Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore: energy transfer, quenching and intracellular pH sensing

Covalent and non-covalent coupling of a Au₁₀₂ nanocluster with a fluorophore: energy transfer, quenching and intracellular pH sensing