Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one of these species, used in the region for traditional medicine and ornamental purposes. Therefore, optimizing alternative micropropagation protocols of tissue culture conditions and secondary metabolite production for these species needs attention. The effect of Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot regeneration in H. pallasi was investigated during this study. The leaf, stem and root parts taken from the seedling germinated under in vitro conditions were used as explants. As a result, the root explants were more efficient compared to other explants in inducing plant regeneration using 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (88.9%) and 1 mg L-1 TDZ treatment (85.7%). The lowest plant regeneration percentage (16.7%) was found in control medium using leaf explants.