Evaluation of Lantana Trifolia Total Extract in Cell Culture: Perspective for Tissue Engineering

Phytotherapy can have several applications, such as anti-inflammatory, analgesic, antinociceptive, antimicrobial, antiviral and tissue regeneration. It is important to characterize the effects of these drugs to verify clinical safety, avoiding possible adverse reactions, like toxicity, digestive discomfort and headache. In this research, the effects of total aqueous and ethanolic extract of Lantana trifolia L was analyzed on Vero cell cultures, seeking to understand how the cellular response to different concentrations (25, 50, 75 and 100 mu l/ml) of the extracts. There were carried out quantitative and qualitative, morphological evaluation, of the cultured cells in the presence of the extracts. The quantitative results showed cell viability dependent on the concentration of test extract. The cellular viability remained above 70%, therefore for all experimental conditions, resulting in non-cytotoxic effects, both for aqueous and ethanolic extracts. With the statistical analysis of the results obtained it was possible to conclude that there was no significant variation in cell viability, that is, although the extract is non-cytotoxic, it does not cause cell proliferation in the medium. The quantitative results are in accordance with the qualitative analyzes.