Highly Purified Top1-Bound DNA Fragments.

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

Topoisomerase 1-DNA cleavage complexes (Top1ccs) form at DNA sites of Top1 activity and are increased by a highly specific anticancer drug (camptothecin, CPT) in living cells. Various methods are available to detect Top1ccs in cultured cells, including protocols based on the use of specific antibodies. Here, we describe a protocol to isolate Top1ccs at high purity, which does not depend on antibodies.

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关键词

Cesium chloride gradient,K+-SDS precipitation,Top1cc (Topoisomerase 1–DNA cleavage complex),Topoisomerase I

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