Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays

•Antibodies to ENA are important in the diagnosis of autoimmune rheumatic diseases.•Many different immunological methods are available to the clinical laboratory.•Diagnostic performance of current immunoassays show good comparability.•Though agreement among assays is good, differences exist in diagnostic accuracy.•Clinicians and laboratory scientists should be aware of these differences.