Recent progress on single-molecule localization microscopy

Super-resolution imaging based on single-molecule localization has been developed for more than a decade.These techniques can break through diffraction limit of fluorescent microscopy and initially im-prove the resolution by an order of magnitude to ～20 nm,by introducing photoactivatable/photo-switching probes and centroid fitting method.As the demand of biological research,the localization precision of single-molecules was further improved by several state-of-the-art methods in the past sev-eral years.This review focuses on the latest developed techniques which have greatly improved the performance of single-molecule localization microscopy,from measurement principle to hardware design.These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells.