Neokomagataea anthophila sp. nov., an osmotolerant acetic acid bacterium isolated in Thailand and emended description of the genus Neokomagataea .

A novel Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium isolated from a sea bean flower [ (Sw.) DC.] collected in Surat Thani Province, Thailand, and designated as AH18 was characterized on the basis of polyphasic taxonomy. The phylogenetic analysis of 16S rRNA gene revealed that strain AH18 represented a member of the genus . In the 16S rRNA gene sequence analysis, the strain's closest phylogenetic neighbour was TBRC 376. The draft genome size of strain AH18 was 2613495 bp, and its DNA G+C content was 52.0 mol%. The strain showed 90.3 and 76.3% pairwise-determined whole-genome average nucleotide identity and 39.8 and 19.6% digital DNA-DNA hybridization values with TBRC 376 and TBRC 7768, respectively. The 16S rRNA gene sequences and phylogenomic analysis revealed that the strain clustered with the members of the genus but was located in a distinct branch closely related to TBRC 376. The predominant cellular fatty acids of the strain were summed feature 8 (C 6 and/or C 7), C and C 2OH (>5%). The major respiratory ubiquinone was Q-10. In addition, strain AH18 was substantiated by differences in several physiological characteristics and by MALDI-TOF profiling. On the basis of the results obtained from phenotypic, chemotaxonomic, phylogenetic and genomic analyses, the strain clearly represented a novel species within the genus , for which the name sp. nov. (AH18=TBRC 2177=NBRC 115156) is proposed. An emended description of the genus is also given.