Sortase-mediated immobilization of Candida antarctica lipase B (CalB) on graphene oxide; comparison with chemical approach

Faezeh Moosavi,Faezeh Ahrari,Gholamreza Ahmadian,Mehdi Mohammadi

Biotechnology Reports（2022）

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摘要

•Sortase A was used for the oriented immobilization of CalB on graphene oxide nanosheets•Random attachment of CalB on GO nanosheets were performed by chemical immobilization•The immobilized CalB were used for the enrichment of omega-3 fatty acids in fish oil•The derivative obtained from oriented immobilization showed improved selectivity

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关键词

sortase,oriented immobilization,graphene oxide

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