Pretreatment Methods for the Determination of Antibiotics Residues in Food Samples and Detected by Liquid Chromatography Coupled with Mass Spectrometry Detectors: A Review.

With the increasing use of antibiotics worldwide, antibiotic monitoring has become a topic of concern. After metabolizing of antibiotics in animals, the metabolites enter the environment through excreta or ingested by the human body via food chain that may exacerbate the emergence of antibiotic resistance and then threaten human's life. This article summarized several analytical methods used for the determination of antibiotics in recent 10 years. Due to the complex matrices and low concentration level of antibiotics in the food samples, a reliable analysis method is required to maximize the recovery rate. Several techniques like solid phase extraction (SPE), dispersive liquid-liquid microextraction (DLLME) and QuEChERS have been frequently used in the pretreatment process for analytes extraction and concentration. After the pretreatment, ultra-high performance liquid chromatography combined with mass spectrometry has been a reliable method for quantitative analysis and is able to determine multiple antibiotics simultaneously. This review also gives an overview about analytical conditions for antibiotics residues in different food samples and their method validation parameters.