Chimerism monitoring using biallelic single nucleotide or insertion/deletion polymorphisms: How many markers to screen?

•Enough markers need to be screened to obtain informative chimerism results.•Analysing too many markers unnecessarily increases the assay’s cost.•There is no research on the required number of biallelic markers to screen.•A flexible simulation framework allows selection of the number of markers.•Simulation results are confirmed by empirical clinical data.