Coupling of multivariate curve resolution-alternating least squares and mechanistic hard models to investigate antibody purification from human plasma using ion exchange chromatography

•Immunoglobulin g purification using two sequential steps by precipitation and ion exchange chromatography procedures.•A combination of MCR-ALS procedure and mechanistic chromatography model is developed.•Estimating of model parameters in the presence of co-eluted interference.•Determining uncertainty based on the new jackknife LOO resampling method.