Alternative Assay Reagents for UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module

Felix Kaspar,Charity S G Ganskow,Lea Eilert,Philipp Klahn,Anett Schallmey

ANALYTICAL CHEMISTRY（2022）

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摘要

This report advises against the use of 5-iodoridine or 5-ethynyluridine as alternative assay reagents in the PUB module, primarily due to their lack of an isosbestic point of phosphorolysis under moderately alkaline conditions.

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关键词

pyro-phosphate,assay,uv-spectroscopic

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