Recording large-scale, cellular-resolution neuronal activity from freely-moving mice

To date, recording of cellular-resolution activity from awake mice has required either head fixation under a microscope or attaching a miniaturized device to the skull, both of which inevitably affect mouse behavior. We demonstrate a new approach for recording thousands of neurons in freely-moving, non-restricted mice during several behavioral tasks using the calcium integrator CaMPARI and a simple optical setup without any optical device attached to the mouse during testing. ### Competing Interest Statement The authors have declared no competing interest.