There Is No Influence of Egg Size on Sex Allocation in Arrhenotokous Lineages of Thrips tabaci Lindeman

Simple Summary How parents control the sex of their offspring greatly differs in the animal kingdom. Two lineages in the Thrips tabaci Lindeman cryptic species complex exhibit arrhenotokous haplodiploidy, which enables parents to influence the sex of offspring by different fertilization mechanisms. In two other haplodiploid species, sex allocation is mediated by egg size. Contrary to the two haplodiploid arthropods with an egg-size-mediated sex allocation mechanism, our study proves that a different mechanism that is independent of egg size regulates sex allocation in T. tabaci. The results presented in this paper raise intriguing questions regarding the evolutionary forces driving egg size and sex allocation in haplodiploids. In addition, our results indicate a significant reduction in egg size with increasing maternal age that cannot be attributed to the resource depletion hypothesis. In two haplodiploid species, sex allocation in young arrhenotokous females is mediated by egg size. We tested if sex allocation is mediated by egg size in two arrhenotokous lineages of the haplodiploid species complex of T. tabaci: L1 and T. We measured the size of eggs produced by very young mothers, 3-5-day-old mothers (just like in the case of Tetranychus urticae) and 7-10-day-old mothers (as in Pezothrips kellyanus). Moreover, we measured the size of eggs oviposited by mothers in their entire lifespan. We found that in T. tabaci, sex allocation is not mediated by egg size. Egg size and gender were independent of maternal age in the L1 lineage, whilst in the T lineage, the observed egg size difference between males and females was only present in the progeny of young females (7-10-day-old mothers). Furthermore, we found that male eggs produced by mated mothers were larger than those produced by virgin mothers in the L1 lineage, but in the T lineage, there were no differences in the size of male eggs produced by mated and virgin mothers. Our results indicate that these two subspecies have different resource allocation strategies in response to maternal mating status.