Slow post-induction specific growth rate enhances recombinant protein expression in Escherichia coli: Pramlintide multimer and ranibizumab production as case studies
Process Biochemistry(2022)
摘要
•There is a correlation between post-induction specific growth rate (SGR) and recombinant protein production.•Slow post-induction SGR improves heterologous protein expression in Escherichia coli (E. coli).•Optimum post-induction SGR varies from protein to protein.
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关键词
Specific growth rate,Post-induction,Exponential feeding,E. coli,Pramlintide,Ranibizumab
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