Estimation of the Genealogical Structure of Vyatka Horse Breed ( Equus ferus caballus ) Using DNA Analysis

The process of forming a genealogical structure in the Vyatka breed makes it relevant to assess its differentiation at the genetic level. As a result of testing 293 Vyatka horses at 17 DNA microsatellite loci, 136 alleles were identified ( A e = 4.11. H o = 0.726) and the genetic differentiation of ten lines was assessed for a number of indicators, including the fixation index F st (0.011–0.134). Sequencing of the mtDNA D-loop region (530 bp) showed the presence of 11 haplogroups with a high level of haplotype variability in family lines. The high level of genetic differentiation of the genealogical structure of the Vyatka horse breed is a prerequisite for successful work to preserve this unique small population.