Structural characterization and immunoregulatory activity of glycoprotein in Lanzhou lily

Lanzhou lily has been confirmed as health care product and medicine because of its great ornamental and edible medicinal values. At present, the research on Lanzhou lily glycoprotein has not been reported in the literature. In this study, the glycoprotein components of Lanzhou lily were extracted and purified, and its structural characterization and immunological activity were studied. The results showed that Lanzhou lily glycoprotein (LGP-1) was linked by O-glycopeptide bonds and had characteristic absorption peaks of carbohydrate and protein. Moreover, LGP-1 possessed great immunological activity. When RAW264.7 was cultured for 48 hours with the glycoprotein concentration of 100 mu g/mL, it could significantly promote cell proliferation and activate RAW264.7 to release NO and TNF-alpha. This confirms that LGP-1 has the potential to become a functional food.