Identification and expression analysis of BnaCNGC family gene in the response to phytohormones, abiotic and biotic stresses in Brassica napus

The cyclic nucleotide-gated ion channel (CNGC) family affects the uptake of Ca2+, regulates the growth and development, pathogen defense, and abiotic stress tolerance in plants. However, the systematic identification, origin, and function of the CNGC gene family has not been performed in Brassica napus. In the present study, we identified 61 putative BnaCNGC genes in the B. napus genome, which are non-randomly localized on 18 chromosomes, and could be classified into five major groups: Group I, II, III, IV-a, and IV-b. Gene structure, multiple sequence alignment, and MEME analysis showed that all the CNGC genes are intron rich and conserved. The expression analysis showed that the BnaCNGC genes have different expression patterns in B. napus, under different phytohormones, abiotic stresses (cold, hot, waterlogging), and infection of Sclerotinia sclerotiorum. Among them, four genes (BnaC03g31050D, BnaC03g31720D, BnaA05g01380D and BnaC04g01250D) from Group I and two genes (BnaCnng45430D and BnaA03g34680D) from Group IV-a, all were strongly induced by SA and infection of S. sclerotiorum, and reduced by cold and heat stresses, suggesting their importance in the abiotic and biotic stress responses in rapeseed. Our comprehensive genome-wide analysis represents a rich data resource for studying the CNGC gene family in B. napus.