Application of barnuf (Pluchea dioscoridis) leaves extract as a natural antioxidant and antimicrobial agent for eggs quality and safety improvement during storage

In this research, the antimicrobial activity of barnuf leaves extract (BLE) was tested against a variety of microbial species, and used for improving the quality and stability of eggs throughout storage. BLE was effective as an antimicrobial agent against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium and Candida albicans. Captured micrographs of P. aeruginosa, S. aureus and C. albicans cells treated with BLE demonstrated significant modifications in cell morphology; after 24 hr of treatment cells were completely lysed and disrupted. The results shows that eggs coated with CMC-6% BLE coating (T5) had significantly higher albumen weight, Haugh Unit, Yolk Index, total phenolic content, total flavonoids content and lower Weight loss, TBARS and total microbial count than those for the other species (p < .05). It is suggested that CMC-BLE coating may be applied before storage to ensure good quality egg protection especially in tropics and most developed nations around the world. Practical applications Fresh eggs are very nutritious, but they can quickly deteriorate due to the lack of carbon dioxide and moisture by pores in the eggshell surface. As a result, using surface coatings and innovative treatment methods to extend shelf life while preserving interior and functional attributes is very successful. The recent research contains ground-breaking information on extending the shelf life of eggs by coating them with CMC incorporated with barnuf leaves extract as an edible coating. This work would aid the egg industry in maintaining the quality of their products as well as extending the shelf life of eggs.