Response of Xanthomonas citri pv. malvacearum Isolates to Cotton Differing in Susceptibility to the Bacterium and Their Predicted Type III Effectors

From 2015 to 2020, 342 isolates of Xanthomonas citri pv. malvacearum (Xcm) were obtained from cotton fields in Texas, including 64 isolates collected from symptomatic cultivars that were thought to be resistant to race 18 strains of Xcm (the predominant race in the United States). Symptoms on highly resistant cultivars prompted concern that a new race of Xcm was present. The 342 isolates were inoculated on a race 18 susceptible (DP 1747NR B2XF) and race 18 resistant (S295) cotton cultivar, and none of the isolates caused blight-type symptoms (water soaking and chlorosis) on 5295, indicating that the B-12 resistant gene was still beneficial for disease management. Four cultivars, varying in their field response to bacterial blight, were inoculated with each of 17 isolates of Xcm, and the incidence of plants exhibiting bacterial blight symptoms averaged 87% for DP 1747NR B2XF, 51% for partially susceptible NG 4936 B3XF, 16% for partially resistant DP 1646 B2XF, and 0% for 5295. Xcm isolates from Texas (11), Georgia (1), and Oklahoma (1) were sequenced, and their type three effectors (T3Es) were predicted. All isolates (Georgia, Oklahoma, and Texas) had the same T3E proteins as previously identified Xcm race 18 isolates (tested for 25 genes), including XopJ. Race 1, 2, 3, and 12 of Xcm included in the comparisons were all missing the Xopi gene. Use of cultivars with the B-12 gene is an effective strategy to manage bacterial blight of cotton.